I'm going to take one again in 2 weeks which will make 6 weeks is it possible to change. I tested negative. Is that Can I take appetite suppressant after my 8 weeks of Oxyelite Pro are done? When can I take all you pro and anti-China trolls on a fishing trip? How can i get a flat stomach in 2 weeks or sooner?
Can I take my nose piercing out after 3 weeks? Can I lose my belly fat in 8 weeks? Best solution Is dexatrim a good appetite suppressant? Answer: Nothing you can take over the counter is good for you when trying to lose weight. Other solutions How long does it take for the appetite suppressant to kick in?
Answer: For me, the appetite suppressant side effect of Strattera kicked in after about 1 week on the medication Answer: Green tea is a natural ingredient appetite suppressant.
No adverse effects have been noted with regards to clinical safety markers eg, blood chemistry. Despite these findings, no scientific evidence is currently available pertaining to the effects of this dietary supplement following a longer term intervention period.
This is true with regards to anthropometric and safety data, as well as other bloodborne variables of interest associated with obesity such as serum lipids and markers of oxidative stress eg, malondialdehyde. Therefore, the purpose of the present study was to determine the effects of OxyELITE Pro on weight loss and associated markers following an eight week intervention, using a randomized, placebo controlled, double blind design.
We hypothesized that subjects in the supplement group would experience more favorable changes in weight Thirty-two recreationally exercise-trained men or women 5. The indicated sample of 32 subjects is similar to many other studies involving weight loss dietary supplements.
Our inclusion of men and women of younger age was done in an attempt to mimic the subject population which represents the market for weight loss dietary supplements. Traditionally, weight loss studies include only young to middle aged obese subjects, typically women who are inactive.
One unique aspect of our study is the inclusion of an equal number of men and women, all who exercise regularly, and most who are not obese. Such a sample has greater generalizability to the target market for weight loss supplements. Subjects were nonsmokers and did not have any cardiovascular or metabolic problems that might affect their response to treatment and their ability to participate.
Health history, drug and dietary supplement usage, and physical activity questionnaires were completed by all subjects to determine eligibility. Concerning human subjects, each was informed of all procedures, potential risks, and benefits associated with the study through both verbal and written form prior to participation. All subjects signed an informed consent form prior be being admitted. During the initial visit to the laboratory, subjects completed the informed consent form, health and physical activity questionnaires.
Subjects were provided with food logs and instructions regarding how to complete these logs during the week prior to beginning their assigned condition and during the final week of the assigned condition please see description below. Subjects received a detailed schedule for the entire study period outlining all pertinent dates of participation.
For both visits to the laboratory pre and post intervention , subjects reported in the morning hours am following a 10 hour overnight fast. Upon arrival, food records were collected and reviewed with subjects. Subjects were then asked to void. Subjects then sat in a chair and rested for 10 minutes. Heart rate via 60 second palpation of the radial artery and blood pressure via auscultation using a two-earpiece stethoscope was then measured and recorded.
A blood sample was then obtained described below. Following this, subjects height, weight, waist and hip circumference, skinfold thickness 7 site using a Lange caliper , and body composition was measured. Both total and regional trunk specific body fat were determined, and fat and fat free mass were calculated. The DEXA assessment was performed by a licensed technician.
These exact procedures were followed for both test days pre and post intervention. Blood was collected from subjects on two different days throughout the course of the study: pre intervention day 1 of the studythe morning of the first day of supplement or placebo use and post intervention the day following the final day of supplementation.
On each occasion, venous blood samples 25 mL were taken from subjects via needle and Vacutainer. All blood samples were collected in a fasted and 10 minute rested state.
Following collection, samples were processed and immediately placed in the refrigerator or the freezer, depending on the sample. A portion of blood samples were sent to Laboratory Corporation of America for analysis of lipid panel, complete blood count, and comprehensive metabolic panel. The complete blood count was determined using an automated cell counter Coulter LH Remaining blood was stored at 70 C until analyzed for malondialdehyde, following the procedures of Jentzsch et al,14 using reagents purchased from Northwest Life Science Specialties Vancouver, WA.
The supplement contained a proprietary blend of caffeine, bauhinia purpurea, bacopa monniera, geranium stem extract 1,3 dimethlyamlamine , cirsium oligophyllum, and rauwolscine extract as the active ingredients. Capsules were produced in accordance with Good Manufacturing Practices and from the same lot number.
The placebo microcrystalline cellulose and OxyELITE Pro capsules were identical in appearance and were dispensed to subjects in identically labeled bottles, at the start of the study and after four weeks.
Neither the subjects nor the investigators involved in data collection were aware of the assigned treatment. Subjects were instructed to use the supplement or placebo in the same manner as suggested on the product label. Specifically, subjects were instructed to Ingest 1 capsule daily for the first three days.
If the single capsule each day is well-tolerated, then, starting on day four, try ingesting an additional capsule, 56 hours after the first capsule. If this is welltolerated, then this will be your dosage throughout the eight week study period. If, however, taking the second capsule causes any adverse effects such as sleeplessness, then you should attempt to ingest 2 capsules at once in the morning, provided that it is well-tolerated. If ingesting 2 capsules at once in the morning is not well-tolerated, then you should revert back to 1 capsule daily in the morning.
Therefore, subjects were provided the option to use either 1 or 2 capsules per day. This was done in an attempt to duplicate the conditions in which individuals would use this dietary supplement in a non-laboratory based setting. Subjects reported their intake to investigators when capsule bottles were returned and compliance to treatment was determined by counting remaining capsules.
For both conditions, capsules were taken with water on an Subjects were instructed to maintain their normal diet inclusive of food and beverages during the eight week study period and to record intake during the week prior to each test day. Diet records were analyzed for total kilocalories, protein, carbohydrate, fat, and a variety of other nutrients Food Processor SQL, version 9. Subjects were asked to maintain their normal physical activity habits and exercise training schedule during the study period, with the exception of the two days 48 hours prior to each test day, in which they were asked not to perform any strenuous exercise.
Subjects were not required to maintain activity logs. The primary outcome measures were also analyzed using a paired t-test comparing pre and post intervention data independently for both supplement and placebo. The data are presented as mean SEM. All analyses were performed using JMP statistical software version 4. Statistical significance was set at P 0.
Subject compliance to treatment, appetite, descriptive characteristics, anthropometric and hemodynamic data are presented in Table 1.
All subjects assigned to the placebo ingested two capsules per day. Of the 16 subjects assigned to the supplement, 11 ingested two capsules per day and five ingested only one capsule per day. These five subjects indicated that the ingestion of two capsules was associated with increased feelings of jitters and sleeplessness.
None of the remaining 11 subjects assigned to the supplement noted any adverse effects of treatment. Due to scheduling conflicts, four subjects three using supplement;. Table 1. Descriptive characteristics, anthropometric and hemodynamic data for 32 men and women assigned to OxyeLITe Pro or placebo for eight weeks. No condition effects noted P. No interactions or main effects were noted for any anthropometric or hemodynamic variable P.
However, when comparing pre and post intervention values for the supplement, significant decreases were noted in body weight, BMI, waist circumference, waist:hip, total body fat percentage, fat mass, fat free mass, and skinfold thickness P , 0. Lipid panel and malondialdehyde data are presented in Table 2. With the exception of malondialdehyde, no interactions or main effects were noted for any lipid specific parameter P. A condition P , 0. When comparing pre and post intervention values for the supplement, significant increases were noted in total cholesterol, HDL-C, and malondialdehyde P , 0.
Complete blood count data are presented in Table 3. No other interactions or main effects were noted for complete blood count data P. Metabolic panel data are presented in Table 4. No other interactions or main effects were noted for metabolic panel data P. Appetite and dietary intake data are presented in Table 5. No other interactions or main effects were noted for dietary data P.
The findings from our investigation indicate that the dietary supplement OxyELITE Pro may assist in weight and body fat loss, while improving selected markers of the blood lipid panel.
At a daily dosage of. Table 2. No other condition effects noted P. Mccarthy et al Table 3. However, the supplement does result in an increase in resting heart rate of approximately six beats per minute. Although this increase in heart rate was not accompanied by a significant increase in systolic or diastolic blood pressure 3 mmHg , it may be wise for hypertensive individuals to avoid use of this supplement, as any increase in these variables may be undesirable.
These data extend our prior work with this supplement. Using an acute laboratory study involving single ingestion of the supplement or placebo in a crossover design, we noted an increase in circulating free fatty acids and glycerol, as well as an increase in resting metabolic rate in men and women.
Our collective findings indicate this to be the case; that is, when comparing pre and post intervention data for subjects in the supplement condition. From an efficacy point of view, the variables with the greatest interest in this investigation are those presented in Table 1.
In terms of anthropometric variables, although no interaction effects were noted, when values were compared for each condition independently from pre to post intervention, we noted significant changes in many variables. For example, body weight, waist circumference, skinfold thickness, and body fat percentage were decreased. However, it should be noted that a small portion of the body weight lost was fat free mass, a common finding in weight loss intervention studies. First, malondialdehyde was increased from pre to post intervention in both conditions.
This was surprising, as lipid peroxidation and oxidative stress are associated with levels of adiposity and an obese state. To the contrary, malondialdehyde was increased slightly in the supplement condition from pre to post intervention despite the loss in body weight and body fat, and more so in the.
OEP dosages were determined in accordance to doses recommended on the label of the OEP: 1 capsule to 70 kg-adult body weight i. Rats were daily supplemented for 4 weeks. Acute data were obtained 30 min after the first OEP administration while chronic data were obtained after 4 weeks supplementation. The same animals were used in both protocols. At the end of the experimental period, rats were anesthetized with an i.
At the end of the protocol, skeletal muscle soleus and gastrocnemius , heart, liver and adrenal glands samples were surgically removed.
Open field test was used to assess spontaneous physical activity as previous published by Rosic et al. At beginning of the test each rat was placed in the center of the arena. Rat movements were recorded by a digital video camera placed centrally above the open field for 5 min and analyzed using AnyMaze software.
Spontaneous physical activity was assessed 30 min after the first OEP administration acute response and 30 min after the last OEP administration at the end of the forth week protocol chronic response.
Exercise capacity was determined by graded treadmill exercise test, a method used for detecting exercise intolerance as previous used by our group [ 20 ]. Forty-eight hours after the adaptation period, rats were placed in the exercise streak and allowed to acclimatize for at least 30 min.
The ETT was performed following the open field test. Rats were placed in individual metabolic cages Tecniplast during 48 h for analysis of food and water consumption as previous published by Berger et al. The first 24 h were used for adaptation and the following 24 h were used to record food and water intake.
Metabolic cage was performed 30 min after the first OEP administration acute response and 30 min after the last OEP administration at the end of the forth week protocol chronic response. The levels of lipid peroxidation were determined using the thiobarbituric-acid reactive substances TBARS spectrophotometric assay based on the reaction between malondialdehyde MDA and triobarbituric acid TBA as previous described by our group Leal et al.
Briefly, the samples were homogeneized with trichoroacetic acid and butylated hydroxytoluene. After being vortexed, the samples were placed in dry bath and then centrifuged. The upper phase was diluted with triobarbituric acid and placed in dry bath for 30 min, and the upper phase was read at nm using a spectrophotometer. Additionally, the advanced oxidation protein products AOPP assay protocol was used to evaluate the oxidation of proteins as previous described [ 22 ].
Briefly, samples were diluted with phosphate buffer solution, potassium iodide KI and acetic acid, vortexed for 6 min and then measured at nm. Total protein content was determined by the Bradford method. A standard curve was constructed using stock solution of transaminases and the substrates.
All samples of transaminases were measured at nm and GGT was measured at nm. Mitochondrial to nuclear DNA ratio was used to estimate mitochondrial copy number in skeletal muscle tissue. Statistical software SPSS version No difference was observed in 4. Effect of OEP supplementation on exercise capacity. OEP, Oxyelite Pro. When rats were daily supplemented for 4 weeks with These data suggest a positive effect of acute OEP supplementation but a negative effect on exercise capacity when OEP is administered continuously for 4 weeks chronic.
Spontaneous physical activity was measured using the open field test Fig. No significant difference was observed among groups in any of the parameters analyzed: total running distance Fig. Although not statistically different from Control group, there was a clear tendency to increase spontaneous physical activity after acute OEP supplementation at dose of Effect of OEP supplementation on spontaneous locomotor activity.
OEP is also a well-known dietary supplement for weight loss. Body weight was analyzed throughout the 4 weeks protocol while water and food intake were analyzed during the first 24 h after the first OEP administration acute and during 24 h after the last week of the chronic administration protocol Table 1.
Body weight was not different among groups at the beginning or at the end of the protocol Table 1. In agreement, no difference in food or water intake was observed among groups after acute administration or after 4 weeks OEP supplementation measured by the metabolic cage Table 1.
Altogether there is no evidence that either acute or chronic OEP supplementation inhibits appetite or aids to decrease body weight at least in normal feed rats. Also, at the end of the chronic protocol 4 weeks , gastrocnemius Control, 4. Cases of acute hepatitis and liver injury were related to the use of OEP, however the amount of OEP ingestion was reported as unknown or at high doses [ 4 , 5 , 23 ]. Effect of OEP supplementation on liver injury markers.
Effect of OEP supplementation on circulating and tissue oxidative stress markers. Control group. Also, red and white gastrocnemius, and heart were similar among groups. However, OEP at both doses decreased lipid peroxidation in soleus muscle Effect of OEP supplementation on mitochondrial biogenesis markers. The findings from our study indicate that a single OEP administration stimulated exercise performance OEP was mainly sold as fat burner and to assist in rapid weight loss.
In the present study we found no difference in body weight and food intake of the animals supplemented with OEP. However, data found in the literature are controversial on this subject. The effect of OEP in reducing body weight and appetite was described with 1 or 2 serving doses during 8 weeks by McCarthy et al. However, it is noteworthy that the subjects of both studies were regularly engaged in physical activity protocols which may have contributed to the weight loss and both studies were financed by USP Labs, the OEP manufacturer.
Also, our results corroborate with data found by Whitehead et al. In our study, OEP showed no significant effect on spontaneous locomotor activity. Although none has yet studied OEP on spontaneous locomotor activity before, Dolan et al. The authors observed that acute DMAA administration induced depressant effects within 0 to 30 min which lasted for 50 to 70 min, but increased locomotor activity after to min.
This apparently disparate data may be attributed to the fact that the dietary supplement OEP has undetermined amount of DMAA and also has caffeine on its formulation, which biased this comparison. Acute OEP administration at both Bloomer et al. However, the authors reported that their results may have been biased due to previously daily use of caffeine throughout the protocol informed by the participants.
Due to the lack of data explaining DMAA effects, these findings with caffeine from literature could at least partially explain our results. Thus, we believe that the stimulatory effect observed after acute OEP administration is more likely due to caffeine in the supplement instead of the DMAA amount.
Oppositely to findings from acute administration, 4 weeks of OEP harmed exercise performance. Vaughan et al. Data from both studies are difficult to compare since they used a cell culture model and doses of OEP that cannot be translated to the recommended doses in humans [ 6 ]. ROS are essential signaling molecules that contribute to up-regulation for the expression of several genes, including genes related to mitochondriogenesis [ 29 ].
There is evidence for an antioxidant role of caffeine. Barcelos et. In addition, we cannot exclude the antioxidant effects of OEP as previous described by McCarthy et al. The authors observed lower plasmatic MDA levels than placebo after OEP supplementation for 8 weeks with 2 serving doses.
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